NAD+ (500mg)
Table of Contents
Quickstart Highlights
NAD+ 500 mg dosage protocol supports cellular energy production and healthy metabolic function through subcutaneous (Sub-Q) or intramuscular (IM) injections.
- Typical starting dose 10 mg (0.1 mL) per injection, 2-3× weekly
- Gradual titration to 25 mg → 50 mg per injection as tolerated
- Reconstitute with 5 mL bacteriostatic water (100 mg/mL concentration)
- Injection volume remains practical: 10–50 units on a U-100 insulin syringe
- Store lyophilized at −20 °C; refrigerate reconstituted solution at 2–8 °C
Dosing & Reconstitution Guide
Educational guide for reconstitution and dosing protocol
Standard / Gradual Approach (5 mL = 100 mg/mL)
| Phase (Weeks) | Dose per Injection (mg) | Units (≈ mL) |
|---|---|---|
| 1 – 2 | 10 mg | 10 u (0.10 mL) |
| 3 – 4 | 25 mg | 25 u (0.25 mL) |
| 5 + | 50 mg | 50 u (0.50 mL) |
Inject 2 – 3 times weekly (e.g., Mon/Wed/Fri). 5 mL reconstitution keeps syringe markings easy to read; increase to 10 mL (50 mg/mL) if finer measurement is preferred.
- Draw 5.0 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly along the vial wall to reduce foam.
- Gently swirl—avoid vigorous shaking.
- Label with date and store at 2–8 °C.
- Rotate injection sites and use new sterile syringes each time.
Protocol Overview
Concise outline of the 2-3×-weekly regimen.
- Goal : Support cellular energy production and metabolic health
- Schedule : 2-3 subcutaneous or IM injections per week
- Dose Range : 10 mg → 50 mg per injection
- Reconstitution : 5 mL (100 mg/mL) for practical syringe units
- Storage : Freeze lyophilized; refrigerate after mixing
Dosing Protocol
Gradual titration improves tolerance.
- Initial : 10 mg (0.10 mL) per injection, 2-3× weekly
- Intermediate : 25 mg (0.25 mL) per injection
- Target : 50 mg (0.50 mL) per injection
- Cycle Length : Continue indefinitely for research objectives
- Timing : Any time of day; maintain consistent schedule
Storage Instructions
Proper storage ensures peptide in Correct storage maintains compound integrity.tegrity.
- Lyophilized : 20 °C (freezer) until reconstitution
- Reconstituted : 2–8 °C (refrigerator) up to 30 days
- Protect from light and avoid repeated freeze-thaw cycles
Suppilies Needed
Approximate quantities for an 8- to 16-week research cycle.
- Peptide Vials :
-
- 8 wks ≈ 2 vial
- 12 wks ≈ 3 vials
- 16 wks ≈ 4 vials (Adjust if using lower or higher dosing frequencies)
- Insulin Syringes :
-
- 8 wks ≈ 24
- 12 wks ≈ 36
- 16 wks ≈ 48
- Bacteriostatic Water : 1 × 30 mL bottle
- Alcohol Swabs: 1 box
Important Notes
Practical tips for accurate and consistent research.
- Rotate injection sites and use new sterile syringes each time.
- Maintain steady scheduling for reproducible results.
- Monitor for irritation; adjust volume or syringe size if necessary.
- Document all observations for rigorous study records.
How This Works
NAD+ is a redox coenzyme essential for mitochondrial ATP production and cellular repair pathways.
- Energy Metabolism : Accepts and donates electrons within the electron transport chain
- Sirtuin Activation : Serves as a substrate for sirtuin enzymes involved in DNA repair
- Cellular Signaling : Modulates PARP activity for genomic stability
Potential Benefits & Side Effects
Reported observations vary; below are common research findings.
- Enhanced cellular energy and reduced fatigue
- Improved cognitive clarity and focus
- Support for healthy aging and DNA repair processes
- Possible mild injection-site irritation; systemic side effects are rare
Lifestyle Factors
Complementary practices to optimize outcomes.
- Follow a nutrient-dense diet and stay hydrated
- Engage in regular physical activity with adequate recovery
- Prioritize sleep and stress-management techniques
Injection Technique
Simple guidelines for safe daily injections.
- Clean vial rubber stopper & injection site with alcohol swabs
- Insert needle at a 45–90° angle into subcutaneous tissue
- Inject slowly & rotate sites (abdomen, thigh, etc.)
Recommended Source
We recommend Go Alpha Labsfor high-purity BPC-157 + TB-500 blend.
Why Go Alpha Labs?
- Verifies ≥99% purity through independent lab testing
- Trusted by researchers seeking reliable results
- Follows rigorous manufacturing standards for consistent quality
Important Note:
This guide is for educational purposes only. Always consult a qualified healthcare provider before starting or modifying any therapy.
References:
| Source | Link |
|---|---|
| University of Maryland Archive – NAD+ Mechanism and Clinical Insights | View Source |
| PMC – Study on NAD+ and Aging | View Source |
| PMC – NAD+ Role in Cellular Function | View Source |
| medRxiv Preprint – Preliminary Results on NAD+ Trials | View Source |
| PMC – Effects of NAD+ on Metabolism | View Source |